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antibodies to flag  (Proteintech)


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    Structured Review

    Proteintech antibodies to flag
    Antibodies To Flag, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 433 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag+polyclonal+antibody/IkB+alpha+Polyclonal+antibody/pm41922353-263-112-111
    Average 96 stars, based on 433 article reviews
    antibodies to flag - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Plasma membrane-associated calcium signaling regulates arsenate tolerance in Arabidopsis.
    Article Snippet: Arsenate [As(V)] is a metalloid with heavy metal properties and is widespread in many environments.. Dietary intake of food derived from arsenate-contaminated plants constitutes a major fraction of the potentially health-threatening human exposure to arsenic.. However, the mechanisms underlying how plants respond to arsenate stress and regulate the function of relevant transporters are poorly understood.

    Protein Concentration:

    Article Title: Structural basis for the ligand-dependent activation of heterodimeric AHR-ARNT complex
    Article Snippet: .. After the protein concentration measurement for each sample, 40 mg of supernatant was saved as input for western blot using an anti-Flag polyclonal antibody (Proteintech, 20543-1-AP), anti-Myc polyclonal antibody (Sangon Biotech, D155014), and Beta Actin Monoclonal Antibody (Proteintech, 66009-1-Ig). .. Immunoprecipitation was performed with the supernatant and 40 μl of anti-Flag affinity gel suspension (Beyotime, P2271) according to the manufacturer’s instructions, followed by western blot using the anti-Flag and anti-Myc antibodies.

    Article Title: Structural basis for the ligand-dependent activation of heterodimeric AHR-ARNT complex.
    Article Snippet: .. After the protein concentration measurement for each sample, 40mg of supernatant was saved as input for western blot using an anti-Flag polyclonal antibody (Proteintech, 20543-1-AP), anti-Myc polyclonal antibody (Sangon Biotech, D155014), and Beta Actin Monoclonal Antibody (Proteintech, 66009-1-Ig). .. Immunoprecipitation was performed with the supernatant and 40μl of anti-Flag affinity gel suspension (Beyotime, P2271) according to the manufacturer’s instructions, followed by western blot using the anti-Flag and anti-Myc antibodies.

    Western Blot:

    Article Title: Structural basis for the ligand-dependent activation of heterodimeric AHR-ARNT complex
    Article Snippet: .. After the protein concentration measurement for each sample, 40 mg of supernatant was saved as input for western blot using an anti-Flag polyclonal antibody (Proteintech, 20543-1-AP), anti-Myc polyclonal antibody (Sangon Biotech, D155014), and Beta Actin Monoclonal Antibody (Proteintech, 66009-1-Ig). .. Immunoprecipitation was performed with the supernatant and 40 μl of anti-Flag affinity gel suspension (Beyotime, P2271) according to the manufacturer’s instructions, followed by western blot using the anti-Flag and anti-Myc antibodies.

    Article Title: Structural basis for the ligand-dependent activation of heterodimeric AHR-ARNT complex.
    Article Snippet: .. After the protein concentration measurement for each sample, 40mg of supernatant was saved as input for western blot using an anti-Flag polyclonal antibody (Proteintech, 20543-1-AP), anti-Myc polyclonal antibody (Sangon Biotech, D155014), and Beta Actin Monoclonal Antibody (Proteintech, 66009-1-Ig). .. Immunoprecipitation was performed with the supernatant and 40μl of anti-Flag affinity gel suspension (Beyotime, P2271) according to the manufacturer’s instructions, followed by western blot using the anti-Flag and anti-Myc antibodies.



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    Protein expression of soluble TNALP-Flag with single- and double-site homodimeric mutations. Purified WT or mutated TNALP-Flag using Flag-immunoprecipitation with Flag-peptide elution from six replicate transfection experiments. The elutions were pooled together and immunoblotted with ProteinSimple immunoblotting assay (Bio-Techne) with rabbit <t>polyclonal</t> <t>anti-Flag</t> antibody. Residual Flag-peptide in the elutions was used as a loading control. EV: empty vector control. TNALP, tissue-nonspecific alkaline phosphatase.
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    Proteintech flag f3165 m2
    Protein expression of soluble TNALP-Flag with single- and double-site homodimeric mutations. Purified WT or mutated TNALP-Flag using Flag-immunoprecipitation with Flag-peptide elution from six replicate transfection experiments. The elutions were pooled together and immunoblotted with ProteinSimple immunoblotting assay (Bio-Techne) with rabbit <t>polyclonal</t> <t>anti-Flag</t> antibody. Residual Flag-peptide in the elutions was used as a loading control. EV: empty vector control. TNALP, tissue-nonspecific alkaline phosphatase.
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    Image Search Results


    Protein expression of soluble TNALP-Flag with single- and double-site homodimeric mutations. Purified WT or mutated TNALP-Flag using Flag-immunoprecipitation with Flag-peptide elution from six replicate transfection experiments. The elutions were pooled together and immunoblotted with ProteinSimple immunoblotting assay (Bio-Techne) with rabbit polyclonal anti-Flag antibody. Residual Flag-peptide in the elutions was used as a loading control. EV: empty vector control. TNALP, tissue-nonspecific alkaline phosphatase.

    Journal: The Journal of Biological Chemistry

    Article Title: N-linked glycosylation plays an essential role in the stability and function of tissue-nonspecific alkaline phosphatase

    doi: 10.1016/j.jbc.2025.111092

    Figure Lengend Snippet: Protein expression of soluble TNALP-Flag with single- and double-site homodimeric mutations. Purified WT or mutated TNALP-Flag using Flag-immunoprecipitation with Flag-peptide elution from six replicate transfection experiments. The elutions were pooled together and immunoblotted with ProteinSimple immunoblotting assay (Bio-Techne) with rabbit polyclonal anti-Flag antibody. Residual Flag-peptide in the elutions was used as a loading control. EV: empty vector control. TNALP, tissue-nonspecific alkaline phosphatase.

    Article Snippet: Rabbit polyclonal anti-Flag (F7425, Merck, lot 0000120996) or mouse monoclonal anti-Flag M2 (F3165, Merck, lot 0000405712) antibody and mouse anti-actin monoclonal antibody (ACTN05, Invitrogen, lot WI3205973) were used for TNALP-Flag as well as Flag-peptide and actin detection, respectively.

    Techniques: Expressing, Purification, Immunoprecipitation, Transfection, Western Blot, Control, Plasmid Preparation

    Cellular retention of TNALP with N271Q single- and double-site homodimeric mutations. Detection of TNALP-Flag in cell lysates and in cell supernatant (extracellular) of N271Q mutants 16 h post-transfection and culture with 1 μM MG-132. The cells were lysed with RIPA buffer and briefly sonicated. The extracellular TNALP-Flag was purified from cell medium using Flag-immunoprecipitation with Flag-peptide. Immunoblotting was performed with Protein Simple immunoblotting assay (Bio-Techne) with mouse monoclonal anti-Flag antibody. Residual Flag-peptide in the elutions was used as a loading control for extracellular TNALP-Flag. Actin was used as a loading control for cell lysates with mouse monoclonal anti-actin antibody. The image is representative of one independent experiment. EV, empty vector control; TNALP, tissue-nonspecific alkaline phosphatase.

    Journal: The Journal of Biological Chemistry

    Article Title: N-linked glycosylation plays an essential role in the stability and function of tissue-nonspecific alkaline phosphatase

    doi: 10.1016/j.jbc.2025.111092

    Figure Lengend Snippet: Cellular retention of TNALP with N271Q single- and double-site homodimeric mutations. Detection of TNALP-Flag in cell lysates and in cell supernatant (extracellular) of N271Q mutants 16 h post-transfection and culture with 1 μM MG-132. The cells were lysed with RIPA buffer and briefly sonicated. The extracellular TNALP-Flag was purified from cell medium using Flag-immunoprecipitation with Flag-peptide. Immunoblotting was performed with Protein Simple immunoblotting assay (Bio-Techne) with mouse monoclonal anti-Flag antibody. Residual Flag-peptide in the elutions was used as a loading control for extracellular TNALP-Flag. Actin was used as a loading control for cell lysates with mouse monoclonal anti-actin antibody. The image is representative of one independent experiment. EV, empty vector control; TNALP, tissue-nonspecific alkaline phosphatase.

    Article Snippet: Rabbit polyclonal anti-Flag (F7425, Merck, lot 0000120996) or mouse monoclonal anti-Flag M2 (F3165, Merck, lot 0000405712) antibody and mouse anti-actin monoclonal antibody (ACTN05, Invitrogen, lot WI3205973) were used for TNALP-Flag as well as Flag-peptide and actin detection, respectively.

    Techniques: Transfection, Sonication, Purification, Immunoprecipitation, Western Blot, Control, Plasmid Preparation